PA5-31388

antibody from Invitrogen Antibodies

Targeting: WDR19 DYF-2, FAP66, FLJ23127, IFT144, KIAA1638, NPHP13, ORF26, Oseg6, Pwdmp

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31388

Provider

Invitrogen Antibodies

Product name

WDR19 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WDR19 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate and D) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a WDR19 polyclonal antibody (Product # PA5-31388) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WDR19 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WDR19 Polyclonal Antibody (Product # PA5-31388) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using WDR19 Polyclonal Antibody (Product # PA5-31388). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 5% SDS PAGE. WDR19 Polyclonal Antibody (Product # PA5-31388) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using WDR19 (Product # PA5-31388) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WDR19 Polyclonal Antibody detects WDR19 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. WDR19 Polyclonal Antibody (Product # PA5-31388) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.