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LearnPA5-31388
antibody from Invitrogen Antibodies
Targeting: WDR19
DYF-2, FAP66, FLJ23127, IFT144, KIAA1638, NPHP13, ORF26, Oseg6, Pwdmp
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- PA5-31388 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WDR19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain. Predicted reactivity: Mouse (80%), Rat (80%), Rhesus Monkey (95%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WDR19 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate and D) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a WDR19 polyclonal antibody (Product # PA5-31388) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WDR19 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WDR19 Polyclonal Antibody (Product # PA5-31388) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WDR19 Polyclonal Antibody (Product # PA5-31388). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 5% SDS PAGE. WDR19 Polyclonal Antibody (Product # PA5-31388) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using WDR19 (Product # PA5-31388) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WDR19 Polyclonal Antibody detects WDR19 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. WDR19 Polyclonal Antibody (Product # PA5-31388) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
