PA5-96363
antibody from Invitrogen Antibodies
Targeting: MTREX
Dob1, fSAP118, KIAA0052, Mtr4, SKIV2L2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-96363 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SKIV2L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MADAFGDELF SVFEGDSTTA AGTKKDKEKD KGKWKGPPGS ADKAGKRFDG KLQSESTNNG KNKRDVDFEG TDEPIFGKKP RIEESITEDL SLADLMPRVK VQSVETVEGC THEVALPAEE DYLPLKPRVG KAAKEYPFIL DAFQREAIQC VDNNQSVLVS AHTSAGKTVC AEYAIALALR EKQRVIFTSP IKALSNQKYR EMYEEFQDVG LMTGDVTINP TASCLVMTTE ILRSMLYRGS EVMREVAWVI FDEIHYMRDS ERGVVWEETI ILLPDNVHYV FLSATIPNAR QFAEWICHLH KQPC; Positive Samples: HeLa, 22RV1, 293T, U-87MG, Mouse testis, Mouse spleen; Cellular Location: Nucleus, nucleolus, nucleoplasm
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.58 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SKIV2L2 in extracts of various cell lines. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of SKIV2L2 in 300 μg extracts of 293T cells. Samples were precipitated with 3 μg SKIV2L2 Polyclonal antibody (Product # PA5-96363). Western blot was performed from the immunoprecipitate using SKIV2L2 Polyclonal antibody (Product # PA5-96363) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded human breast cancer using. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded mouse testis. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded rat testis. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded rat testis. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.