PA5-96363

antibody from Invitrogen Antibodies

Targeting: MTREX Dob1, fSAP118, KIAA0052, Mtr4, SKIV2L2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96363

Provider

Invitrogen Antibodies

Product name

SKIV2L2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MADAFGDELF SVFEGDSTTA AGTKKDKEKD KGKWKGPPGS ADKAGKRFDG KLQSESTNNG KNKRDVDFEG TDEPIFGKKP RIEESITEDL SLADLMPRVK VQSVETVEGC THEVALPAEE DYLPLKPRVG KAAKEYPFIL DAFQREAIQC VDNNQSVLVS AHTSAGKTVC AEYAIALALR EKQRVIFTSP IKALSNQKYR EMYEEFQDVG LMTGDVTINP TASCLVMTTE ILRSMLYRGS EVMREVAWVI FDEIHYMRDS ERGVVWEETI ILLPDNVHYV FLSATIPNAR QFAEWICHLH KQPC; Positive Samples: HeLa, 22RV1, 293T, U-87MG, Mouse testis, Mouse spleen; Cellular Location: Nucleus, nucleolus, nucleoplasm

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.58 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SKIV2L2 in extracts of various cell lines. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SKIV2L2 in 300 μg extracts of 293T cells. Samples were precipitated with 3 μg SKIV2L2 Polyclonal antibody (Product # PA5-96363). Western blot was performed from the immunoprecipitate using SKIV2L2 Polyclonal antibody (Product # PA5-96363) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded human breast cancer using. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded mouse testis. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded rat testis. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SKIV2L2 in paraffin-embedded rat testis. Samples were incubated with SKIV2L2 Polyclonal antibody (Product # PA5-96363) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.