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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-38085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-EEF2 (Thr56) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Rapamycin Extends Life Span in ApcMin/+ Colon Cancer FAP Model.
Parihar M, Dodds SG, Hubbard G, Javors MA, Strong R, Hasty P, Sharp ZD
Clinical colorectal cancer 2021 Mar;20(1):e61-e70
Clinical colorectal cancer 2021 Mar;20(1):e61-e70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-eEF2 pThr56 in extracts from NIH-3T3 cells treated with serum (10%, 30min) using a Phospho-eEF2 pThr56 polyclonal antibody (Product # PA5-38085).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Forskolin (10uM for 60 min) (Lane 2), NIH/3T3 (Lane 3), 3T3-L1 (Lane 4), Jurkat (Lane 5) and T98G (Lane 6). The blot was probed with Anti-Phospho-EEF2 (Thr56) Polyclonal Antibody (Product # PA5-38085, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 95 kDa band corresponding to Phospho-EEF2 (Thr56) was observed across the cell lines tested and was enhanced upon Forskolin treatment in Hep G2 cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-eEF2 pThr56 in HUVEC cells using a Phospho-eEF2 pThr56 polyclonal antibody (Product # PA5-38085).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-eEF2 pThr56 in paraffin-embedded human breast carcinoma using a Phospho-eEF2 pThr56 polyclonal antibody (Product # PA5-38085).
