702808

antibody from Invitrogen Antibodies

Targeting: EEF2 EEF-2, EF2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

702808

Provider

Invitrogen Antibodies

Product name

Phospho-EEF2 (Thr56) Recombinant Rabbit Monoclonal Antibody (17H31L21)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Pig, Cat, Bovine.

Antibody clone number

17H31L21

Concentration

0.5 mg/mL

References

Submitted references

Intrarenal arteriosclerosis and telomere attrition associate with dysregulation of the cholesterol pathway.

De Vusser K, Winckelmans E, Martens D, Lerut E, Kuypers D, Nawrot T, Naesens M
Aging 2020 Apr 30;12(9):7830-7847

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Characterization of proposed human B-1 cells reveals pre-plasmablast phenotype.

Covens K, Verbinnen B, Geukens N, Meyts I, Schuit F, Van Lommel L, Jacquemin M, Bossuyt X
Blood 2013 Jun 27;121(26):5176-83

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), T98G (Lane 2), 3T3-L1 (Lane 3), 3T3-L1 treated with Forskolin (100 µM for 60 mins) (Lane 4), Hep G2 (Lane 5), Hep G2 treated with Forskolin (100 µM for 60 mins) (Lane 6), NIH/3T3 (Lane 7) and NIH/3T3 treated with Forskolin (100 µM for 60 mins) (Lane 8). The blots were probed with Anti-EEF2 pT56 Recombinant Rabbit Monoclonal Antibody (Product # 702808, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 95 kDa band corresponding to EEF2 pT56 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).