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- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 20107-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20107-1-AP, RRID:AB_10950401
- Product name
- EEF2 antibody
- Antibody type
- Polyclonal
- Description
- EEF2 antibody (Cat. #20107-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ER.
Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation.
Discovery of papyifuran A as an unusual cembrane diterpenoid from Boswellia papyrifera resin reveals EEF2 as a potent new drug target for fibrosis of multiple organs.
Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissection.
OASL enhances mRNA translation and reprograms lipid metabolism to promote cancer progression.
N6-methyladenosine triggers renal fibrosis via enhancing translation and stability of ZEB2 mRNA.
IGF2BP1 phosphorylation in the disordered linkers regulates ribonucleoprotein condensate formation and RNA metabolism.
Multi-omics with dynamic network biomarker algorithm prefigures organ-specific metastasis of lung adenocarcinoma.
iTRAQ proteomics analysis of placental tissue with gestational diabetes mellitus.
The noncanonical RNA-binding protein RAN stabilizes the mRNA of intranuclear stress granule assembly factor G3BP1 in nasopharyngeal carcinoma.
Diphthamide deficiency promotes association of eEF2 with p53 to induce p21 expression and neural crest defects.
PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis.
A molecular network of conserved factors keeps ribosomes dormant in the egg.
Epitranscriptic regulation of HRAS by N(6)-methyladenosine drives tumor progression.
miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.
RNA m(6)A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.
eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.
Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.
Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.
Mihailovic M, Anisimova AS, Erte B, Zhan N, Styliara I, Dagdas Y, Karagöz GE
The EMBO journal 2026 May;45(9):3252-3275
The EMBO journal 2026 May;45(9):3252-3275
Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation.
Oksanen M, Mastropasqua F, Mazan-Mamczarz K, Martindale JL, Ye X, Arora A, Banskota N, Gorospe M, Tammimies K
Nucleic acids research 2026 Feb 5;54(4)
Nucleic acids research 2026 Feb 5;54(4)
Discovery of papyifuran A as an unusual cembrane diterpenoid from Boswellia papyrifera resin reveals EEF2 as a potent new drug target for fibrosis of multiple organs.
Sura MB, Zhou Y, Li J, Cheng Y
Acta pharmaceutica Sinica. B 2025 Jun;15(6):3181-3195
Acta pharmaceutica Sinica. B 2025 Jun;15(6):3181-3195
Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissection.
Liu X, Zhao L, Liu D, Zhao L, Tuo Y, Peng Q, Huang F, Song Z, Niu C, He X, Xu Y, Wan J, Zhu P, Jian Z, Guo J, Liu Y, Lu J, Liang S, Zheng S
Acta pharmaceutica Sinica. B 2025 Jul;15(7):3663-3684
Acta pharmaceutica Sinica. B 2025 Jul;15(7):3663-3684
OASL enhances mRNA translation and reprograms lipid metabolism to promote cancer progression.
Li SB, Yuan L, Zhu QY, Zhang G, Liu YT, Gao MH, Han F, Lin ZR, Zhang H, Tang LQ, Sun L, He QY, Song EW, Young LS, Li YC, Mai HQ, Zeng MS, Zhong Q
Cell reports 2025 Jul 22;44(7):115901
Cell reports 2025 Jul 22;44(7):115901
N6-methyladenosine triggers renal fibrosis via enhancing translation and stability of ZEB2 mRNA.
Cai Y, Zhou J, Xu A, Huang J, Zhang H, Xie G, Zhong K, Wu Y, Ye P, Wang H, Niu H
The Journal of biological chemistry 2024 Sep;300(9):107598
The Journal of biological chemistry 2024 Sep;300(9):107598
IGF2BP1 phosphorylation in the disordered linkers regulates ribonucleoprotein condensate formation and RNA metabolism.
Hornegger H, Anisimova AS, Muratovic A, Bourgeois B, Spinetti E, Niedermoser I, Covino R, Madl T, Karagöz GE
Nature communications 2024 Oct 20;15(1):9054
Nature communications 2024 Oct 20;15(1):9054
Multi-omics with dynamic network biomarker algorithm prefigures organ-specific metastasis of lung adenocarcinoma.
Zhang X, Xiao K, Wen Y, Wu F, Gao G, Chen L, Zhou C
Nature communications 2024 Nov 14;15(1):9855
Nature communications 2024 Nov 14;15(1):9855
iTRAQ proteomics analysis of placental tissue with gestational diabetes mellitus.
Yin X, Yang F, Lin J, Hu Q, Tang X, Yin L, Yan X, Zhuang H, Ma G, Shen L, Zhao D
Acta diabetologica 2024 Dec;61(12):1589-1601
Acta diabetologica 2024 Dec;61(12):1589-1601
The noncanonical RNA-binding protein RAN stabilizes the mRNA of intranuclear stress granule assembly factor G3BP1 in nasopharyngeal carcinoma.
Yang PY, Yang Z, Lv J, Jiang PY, Quan TQ, Huang ZH, Xu XD, Guo R, Wei D, Sun Y
The Journal of biological chemistry 2024 Dec;300(12):107964
The Journal of biological chemistry 2024 Dec;300(12):107964
Diphthamide deficiency promotes association of eEF2 with p53 to induce p21 expression and neural crest defects.
Shi Y, Huang D, Song C, Cao R, Wang Z, Wang D, Zhao L, Xu X, Lu C, Xiong F, Zhao H, Li S, Zhou Q, Luo S, Hu D, Zhang Y, Wang C, Shen Y, Su W, Wu Y, Schmitz K, Wei S, Song W
Nature communications 2024 Apr 26;15(1):3301
Nature communications 2024 Apr 26;15(1):3301
PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis.
Chen W, He Q, Liu J, Li N, Xiao K, Chen H
Carcinogenesis 2023 Jun 24;44(4):328-340
Carcinogenesis 2023 Jun 24;44(4):328-340
A molecular network of conserved factors keeps ribosomes dormant in the egg.
Leesch F, Lorenzo-Orts L, Pribitzer C, Grishkovskaya I, Roehsner J, Chugunova A, Matzinger M, Roitinger E, Belačić K, Kandolf S, Lin TY, Mechtler K, Meinhart A, Haselbach D, Pauli A
Nature 2023 Jan;613(7945):712-720
Nature 2023 Jan;613(7945):712-720
Epitranscriptic regulation of HRAS by N(6)-methyladenosine drives tumor progression.
Pan Y, Gu Y, Liu T, Zhang Q, Yang F, Duan L, Cheng S, Zhu X, Xi Y, Chang X, Ye Q, Gao S
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 4;120(14):e2302291120
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 4;120(14):e2302291120
miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.
Li W, Cui X, Qi A, Yan L, Wang T, Li B
Pathology, research and practice 2019 Nov;215(11):152636
Pathology, research and practice 2019 Nov;215(11):152636
RNA m(6)A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.
Lin X, Chai G, Wu Y, Li J, Chen F, Liu J, Luo G, Tauler J, Du J, Lin S, He C, Wang H
Nature communications 2019 May 6;10(1):2065
Nature communications 2019 May 6;10(1):2065
eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.
Ji C, Xu Q, Guo L, Wang X, Ren Y, Zhang H, Zhu W, Ming Z, Yuan Y, Ren X, Song J, Cheng Y, Yang J, Zhang Y
Cancer letters 2018 Apr 1;418:64-74
Cancer letters 2018 Apr 1;418:64-74
Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.
Lin HP, Zheng DJ, Li YP, Wang N, Chen SJ, Fu YC, Xu WC, Wei CJ
Biomedical microdevices 2016 Jun;18(3):41
Biomedical microdevices 2016 Jun;18(3):41
Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.
Lu Y, Wang J, Dapeng C, Wu D, Cai G, Chen X
BMC nephrology 2016 Apr 1;17:39
BMC nephrology 2016 Apr 1;17:39
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20107-1-AP(EEF2 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using EEF2 antibody 20107-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human stomach cancer using 20107-1-AP(EEF2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human stomach cancer using 20107-1-AP(EEF2 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
