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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [37]
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- Flow cytometry [1]
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- Product number
- MAB1380-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ULBP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 170818
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Trabectedin triggers direct and NK-mediated cytotoxicity in multiple myeloma.
Purine nucleotide metabolism regulates expression of the human immune ligand MICA.
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells.
Adoptively transferred Vγ9Vδ2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model.
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia.
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Stromal cells from human decidua exert a strong inhibitory effect on NK cell function and dendritic cell differentiation.
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
An epithelial cell adhesion molecule- and CD3-bispecific antibody plus activated T-cells can eradicate chemoresistant cancer stem-like pancreatic carcinoma cells in vitro.
NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation.
Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis.
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity.
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion.
HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
Cucè M, Gallo Cantafio ME, Siciliano MA, Riillo C, Caracciolo D, Scionti F, Staropoli N, Zuccalà V, Maltese L, Di Vito A, Grillone K, Barbieri V, Arbitrio M, Di Martino MT, Rossi M, Amodio N, Tagliaferri P, Tassone P, Botta C
Journal of hematology & oncology 2019 Mar 21;12(1):32
Journal of hematology & oncology 2019 Mar 21;12(1):32
Purine nucleotide metabolism regulates expression of the human immune ligand MICA.
McCarthy MT, Moncayo G, Hiron TK, Jakobsen NA, Valli A, Soga T, Adam J, O'Callaghan CA
The Journal of biological chemistry 2018 Mar 16;293(11):3913-3924
The Journal of biological chemistry 2018 Mar 16;293(11):3913-3924
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
Raneros AB, Minguela A, Rodriguez RM, Colado E, Bernal T, Anguita E, Mogorron AV, Gil AC, Vidal-Castiñeira JR, Márquez-Kisinousky L, Bulnes PD, Marin AM, Garay MCG, Suarez-Alvarez B, Lopez-Larrea C
Oncotarget 2017 May 9;8(19):31959-31976
Oncotarget 2017 May 9;8(19):31959-31976
Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells.
Cai X, Caballero-Benitez A, Gewe MM, Jenkins IC, Drescher CW, Strong RK, Spies T, Groh V
Neoplasia (New York, N.Y.) 2017 Jun;19(6):471-482
Neoplasia (New York, N.Y.) 2017 Jun;19(6):471-482
Adoptively transferred Vγ9Vδ2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model.
Zumwalde NA, Sharma A, Xu X, Ma S, Schneider CL, Romero-Masters JC, Hudson AW, Gendron-Fitzpatrick A, Kenney SC, Gumperz JE
JCI insight 2017 Jul 6;2(13)
JCI insight 2017 Jul 6;2(13)
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, Wirth C, Will R, Bott A, Cerwenka A, Wiemann S
Cell death & disease 2017 Aug 3;8(8):e2973
Cell death & disease 2017 Aug 3;8(8):e2973
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Charpak-Amikam Y, Kubsch T, Seidel E, Oiknine-Djian E, Cavaletto N, Yamin R, Schmiedel D, Wolf D, Gribaudo G, Messerle M, Cicin-Sain L, Mandelboim O
Scientific reports 2017 Aug 17;7(1):8661
Scientific reports 2017 Aug 17;7(1):8661
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Son CH, Lee HR, Koh EK, Shin DY, Bae JH, Yang K, Park YS
Scientific reports 2016 Sep 27;6:32470
Scientific reports 2016 Sep 27;6:32470
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer EH, Cerwenka A, Steinle A, Koehl U, Koch J
BMC cancer 2015 May 3;15:351
BMC cancer 2015 May 3;15:351
Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
Enqvist M, Ask EH, Forslund E, Carlsten M, Abrahamsen G, Béziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Önfelt B, Malmberg KJ
Journal of immunology (Baltimore, Md. : 1950) 2015 May 1;194(9):4518-27
Journal of immunology (Baltimore, Md. : 1950) 2015 May 1;194(9):4518-27
Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia.
Baragaño Raneros A, Martín-Palanco V, Fernandez AF, Rodriguez RM, Fraga MF, Lopez-Larrea C, Suarez-Alvarez B
Genes and immunity 2015 Jan-Feb;16(1):71-82
Genes and immunity 2015 Jan-Feb;16(1):71-82
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Cox ST, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal JA, Saudemont A
European journal of immunology 2015 Aug;45(8):2324-34
European journal of immunology 2015 Aug;45(8):2324-34
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Kim GR, Ha GH, Bae JH, Oh SO, Kim SH, Kang CD
Oncology letters 2015 Apr;9(4):1641-1646
Oncology letters 2015 Apr;9(4):1641-1646
Stromal cells from human decidua exert a strong inhibitory effect on NK cell function and dendritic cell differentiation.
Croxatto D, Vacca P, Canegallo F, Conte R, Venturini PL, Moretta L, Mingari MC
PloS one 2014;9(2):e89006
PloS one 2014;9(2):e89006
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
Fielding CA, Aicheler R, Stanton RJ, Wang EC, Han S, Seirafian S, Davies J, McSharry BP, Weekes MP, Antrobus PR, Prod'homme V, Blanchet FP, Sugrue D, Cuff S, Roberts D, Davison AJ, Lehner PJ, Wilkinson GW, Tomasec P
PLoS pathogens 2014 May;10(5):e1004058
PLoS pathogens 2014 May;10(5):e1004058
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Robinet P, Baychelier F, Fontaine T, Picard C, Debré P, Vieillard V, Latgé JP, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5332-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5332-42
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
Soriani A, Iannitto ML, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi MR, Petrucci MT, Cippitelli M, Santoni A
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
An epithelial cell adhesion molecule- and CD3-bispecific antibody plus activated T-cells can eradicate chemoresistant cancer stem-like pancreatic carcinoma cells in vitro.
Umebayashi M, Kiyota A, Koya N, Tanaka H, Onishi H, Katano M, Morisaki T
Anticancer research 2014 Aug;34(8):4509-19
Anticancer research 2014 Aug;34(8):4509-19
NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation.
Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari MC
European journal of immunology 2013 Feb;43(2):550-61
European journal of immunology 2013 Feb;43(2):550-61
Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
Lim SA, Kim TJ, Lee JE, Sonn CH, Kim K, Kim J, Choi JG, Choi IK, Yun CO, Kim JH, Yee C, Kumar V, Lee KM
Cancer research 2013 Apr 15;73(8):2598-607
Cancer research 2013 Apr 15;73(8):2598-607
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
Nielsen N, Ødum N, Ursø B, Lanier LL, Spee P
PloS one 2012;7(2):e31959
PloS one 2012;7(2):e31959
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
Cancer research 2012 Nov 1;72(21):5463-72
Cancer research 2012 Nov 1;72(21):5463-72
Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis.
DelaRosa O, Sánchez-Correa B, Morgado S, Ramírez C, del Río B, Menta R, Lombardo E, Tarazona R, Casado JG
Stem cells and development 2012 May 20;21(8):1333-43
Stem cells and development 2012 May 20;21(8):1333-43
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Glässner A, Eisenhardt M, Krämer B, Körner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbé C, Caillat-Zucman S
PLoS pathogens 2012 Jan;8(1):e1002486
PLoS pathogens 2012 Jan;8(1):e1002486
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
Oncogene 2011 Jun 9;30(23):2622-32
Oncogene 2011 Jun 9;30(23):2622-32
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
Björkström NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaëlsson J, Malmberg KJ, Klingström J, Ahlm C, Ljunggren HG
The Journal of experimental medicine 2011 Jan 17;208(1):13-21
The Journal of experimental medicine 2011 Jan 17;208(1):13-21
The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
Fernández-Messina L, Ashiru O, Agüera-González S, Reyburn HT, Valés-Gómez M
Journal of cell science 2011 Feb 1;124(Pt 3):321-7
Journal of cell science 2011 Feb 1;124(Pt 3):321-7
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Muntasell A, Magri G, Pende D, Angulo A, López-Botet M
Blood 2010 Jun 24;115(25):5170-9
Blood 2010 Jun 24;115(25):5170-9
Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15825-30
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15825-30
Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
International journal of cancer 2009 Jul 1;125(1):212-21
International journal of cancer 2009 Jul 1;125(1):212-21
Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity.
Balsamo M, Scordamaglia F, Pietra G, Manzini C, Cantoni C, Boitano M, Queirolo P, Vermi W, Facchetti F, Moretta A, Moretta L, Mingari MC, Vitale M
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 8;106(49):20847-52
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 8;106(49):20847-52
Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
International journal of cancer 2009 Apr 1;124(7):1594-604
International journal of cancer 2009 Apr 1;124(7):1594-604
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Molecular immunology 2008 Sep;45(15):3917-25
Molecular immunology 2008 Sep;45(15):3917-25
Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD Jr, Barlogie B, van Rhee F
Blood 2008 Feb 1;111(3):1309-17
Blood 2008 Feb 1;111(3):1309-17
Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion.
de Matos CT, Berg L, Michaëlsson J, Felländer-Tsai L, Kärre K, Söderström K
Immunology 2007 Oct;122(2):291-301
Immunology 2007 Oct;122(2):291-301
HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, Barker E
Blood 2007 Aug 15;110(4):1207-14
Blood 2007 Aug 15;110(4):1207-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ULBP-1 in MOLT-4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP-1 Monoclonal Antibody (Catalog # MAB1380, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
