Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-36608 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RhoG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 21 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoG using RhoG polyclonal antibody (Product # PA5-36608) at a dilution of 1:500. Lane 1: A549 whole cell lysate, Lane 2: sp2/0 whole cell lysate, Lane 3: PC12 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RhoG in Lane 1: the Spleen tissue lysate of mouse (40 µg), Lane 2: the Spleen tissue lysate of rat (40 µg), Lane 3: K562 whole cell lysate (40 µg), Lane 4: H1792 whole cell lysate (40 µg). Samples were incubated with RhoG polyclonal antibody (Product # PA5-36608) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched cell extract (30 µg lysate) of Jurkat (Lane 1), THP-1 (Lane 2), MOLT-4 (Lane 3) and Mouse Thymus (Lane 4). The blot was probed with Anti-RhoG Polyclonal Antibody (Product # PA5-36608, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to RhoG was observed in all cell lines and tissue tested along with a non-specific band (*) at ~30 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RhoG in paraffin-embedded human colorectal carcinoma using RhoG polyclonal antibody (Product # PA5-36608) at a dilution of 1:50.