LS-C313094

antibody from LSBio

Targeting: DDX4 VASA

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C313094

Provider

LSBio

Product name

DDX4 / VASA Antibody (aa253-272) LS-C313094

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of DDX4 / VASA antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: Mouse Testis Tissue Lysate. Lane 3: HELA Cell Lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-DDX4/MVH antibody, Western blotting Lane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: HELA Cell Lysate

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-DDX4/MVH antibody, Western blotting Lane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: Mouse Ovary Tissue Lysate

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. ICC: MCF-7 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

DDX4 / VASA antibody. ICC: MCF-7 Cell.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(P): Rat Ovary Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(P): Rat Testis Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(F): Rat Intestine Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody. DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4/MVH antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(P): Rat Testis Tissue.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody. DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4/MVH antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(P): Rat Ovary Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(P): Rat Testis Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

DDX4 / VASA antibody. IHC(F): Rat Intestine Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody. DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4/MVH antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.