AF2675

antibody from Novus Biologicals

Targeting: NTHL1 NTH1, OCTS3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2675

Provider

Novus Biologicals

Product name

Goat Polyclonal NTH1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human NTH1 in Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Transcriptional regulation of the base excision repair pathway by BRCA1.

Saha T, Rih JK, Roy R, Ballal R, Rosen EM
The Journal of biological chemistry 2010 Jun 18;285(25):19092-105

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human NTH1 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human NTH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NTH1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.