MA5-50067

antibody from Invitrogen Antibodies

Targeting: NTHL1 NTH1, OCTS3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

MA5-50067

Provider

Invitrogen Antibodies

Product name

NTHL1 Recombinant Rabbit Monoclonal Antibody (22H10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

22H10

Vial size

100 µL

Concentration

1.5 mg/mL

Storage

-20°C or -80°C if preferred

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis with a NTHL1 monoclonal antibody (Product # MA5-50067) using a dilution of 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.