PA1-16506

antibody from Invitrogen Antibodies

Targeting: NTHL1 NTH1, OCTS3

Antibody data

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Validation data

Product number: PA1-16506 - Provider product page
Provider: Invitrogen Antibodies
Product name: NTHL1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: The target sequence has 93% sequence homology with bovine proteins. Suggested positive control: Hela whole cell lysate, K562 cell extracts, Hela cell extracts, K562 whole cell lysate.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 200 µL
Concentration: 1.1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

NTHL1 protein structure - PA1-16506 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of NTHL1 in formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 1:200 followed by HRP conjugated anti-rabbit secondary antibody with DAB reagent. The sections were further counterstained with hematoxylin for labeling cellular nuclei. The NTH1 antibody generated an expected nuclear cytoplasmic staining of NTH1 protein in colon cancer cells as well as the cells of tumor stroma including cancer associated fibroblasts. The nuclear staining of NTH1 was very strong in a sub-set of colon cancer cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human rectal cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. This representative image at high resolution depicts nuclear-cytoplasmic positivity for NTH1 protein in rectal cancer cells and the goblet cells. The cells of tumor stroma did not develop any immunostaining for this protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human rectal cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. The rectal cancer cells as well as the goblet cells in glandular areas showed nuclear-cytoplasmic positivity for NTH1 protein. The cells of tumor stroma did not develop immunostaining for this protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human kidney cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. The renal cancer cells showed nuclear and cytoplasmic positivity for NTH1 protein, whereas, the tumor stroma was largely negative for immunostaining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human lymph node cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. The representative image shows strong nuclear and cytoplasmic positivity of NTH1 protein in the lymph node cancer cells.