Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- PA1-16506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NTHL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The target sequence has 93% sequence homology with bovine proteins. Suggested positive control: Hela whole cell lysate, K562 cell extracts, Hela cell extracts, K562 whole cell lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 1.1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of NTH1 (37 kDa) from Hela & K562 cell extracts using Product # PA1-16506 (1:500).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NTHL1 in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NTHL1 in formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 1:200 followed by HRP conjugated anti-rabbit secondary antibody with DAB reagent. The sections were further counterstained with hematoxylin for labeling cellular nuclei. The NTH1 antibody generated an expected nuclear cytoplasmic staining of NTH1 protein in colon cancer cells as well as the cells of tumor stroma including cancer associated fibroblasts. The nuclear staining of NTH1 was very strong in a sub-set of colon cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human rectal cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. This representative image at high resolution depicts nuclear-cytoplasmic positivity for NTH1 protein in rectal cancer cells and the goblet cells. The cells of tumor stroma did not develop any immunostaining for this protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human rectal cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. The rectal cancer cells as well as the goblet cells in glandular areas showed nuclear-cytoplasmic positivity for NTH1 protein. The cells of tumor stroma did not develop immunostaining for this protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human kidney cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. The renal cancer cells showed nuclear and cytoplasmic positivity for NTH1 protein, whereas, the tumor stroma was largely negative for immunostaining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NTHL1 in formalin-fixed paraffin-embedded tissue section of human lymph node cancer. Samples were incubated in NTHL1 polyclonal antibody (Product # PA1-16506) using a dilution of 5 µg/mL. The representative image shows strong nuclear and cytoplasmic positivity of NTH1 protein in the lymph node cancer cells.