PA5-28170

antibody from Invitrogen Antibodies

Targeting: UGDH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28170

Provider

Invitrogen Antibodies

Product name

UGDH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse liver, rat liver, UGDH-transfected 293T.

Concentration

1.36 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UGDH using 30 µg HepG2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a UGDH polyclonal antibody (Product # PA5-28170) at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of UGDH was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a UGDH Polyclonal Antibody (Product # PA5-28170) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using UGDH Polyclonal Antibody (Product # PA5-28170). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. UGDH Polyclonal Antibody (Product # PA5-28170) diluted at 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

UGDH Polyclonal Antibody detects UGDH protein by Western blot analysis. A. 50 µg Rat liver lysate/extract.7.5 % SDS-PAGE. UGDH Polyclonal Antibody (Product # PA5-28170) dilution: 1:2,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

UGDH Polyclonal Antibody detects UGDH protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UGDH stained by UGDH Polyclonal Antibody (Product # PA5-28170) diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

UGDH Polyclonal Antibody detects UGDH protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. UGDH Polyclonal Antibody (Product # PA5-28170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.