H00030844-M01

antibody from Abnova Corporation

Targeting: EHD4 PAST4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00030844-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00030844-M01, RRID:AB_425962

Product name

EHD4 monoclonal antibody (M01), clone 1B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant EHD4.

Antigen sequence

MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRK
VLPLEEAYRFHEFHSPALEDADFENKPMILLVGQY
STGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMY
GETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFM
CSQLPNQVLKSISVIDSPGILSGEKQRISRGYDFC
QVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAF
RGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGK
VINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQD
LFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIIS
YLKKEMPSVFGKENKKRELISRLPEIYIQLQREYQ
ISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAV
DNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGT
TEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELF
YTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKL
ADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLP
PHLVPPSHRKSLPKAD


Isotype

IgG

Antibody clone number

1B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

EHD4 monoclonal antibody (M01), clone 1B8 Western Blot analysis of EHD4 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EHD4 expression in transfected 293T cell line by EHD4 monoclonal antibody (M01), clone 1B8.Lane 1: EHD4 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to EHD4 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol