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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX83903 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83903, RRID:AB_10732163
- Product name
- Profilin 1 antibody [1D5]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-PFN1 mouse monoclonal antibody (GTX83903) immunofluorescent staining of COS7 cells transiently transfected with PFN1
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PFN1 mouse monoclonal antibody. (GTX83903, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PFN1 mouse monoclonal antibody. (GTX83903, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody(GTX83903),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC202338 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PFN1 antibody(GTX83903), and then analyzed by flow cytometry.
