NB100-426

antibody from Novus Biologicals

Targeting: PDS5A KIAA0648, PIG54, SCC-112

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-426

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-426, RRID:AB_10001184

Product name

Rabbit Polyclonal SCC112 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SCC112 Antibody [NB100-426] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SCC112 Antibody [NB100-426] - Detection of human SCC-112 in Western Blot and immunoprecipitation, using NB100-426. Samples: HeLa nuclear extracts. Exposure time of less than 5 minutes.