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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-27389 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arylsulfatase E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASE using 30 µg of A) 293T (B) H1299 and C) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ASE polyclonal antibody (Product # PA5-27389) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Arylsulfatase E Polyclonal Antibody (Product # PA5-27389). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Arylsulfatase E Polyclonal Antibody (Product # PA5-27389) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
