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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-21687 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STAR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562, mouse kidney, STAR-transfected 293T. Predicted reactivity: Mouse (90%), Rat (89%), Dog (93%), Cat (89%), Pig (90%), Sheep (86%), Rhesus Monkey (98%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Silencing of the transcriptional factor ZEB1 alters the steroidogenic pathway, and increases the concentration of testosterone and DHT in DU145 cells.
Menaquinone-4 enhances testosterone production in rats and testis-derived tumor cells.
Herrera D, Orellana-Serradell O, Villar P, Torres MJ, Paciucci R, Castellón EA, Contreras HR
Oncology reports 2019 Feb;41(2):1275-1283
Oncology reports 2019 Feb;41(2):1275-1283
Menaquinone-4 enhances testosterone production in rats and testis-derived tumor cells.
Ito A, Shirakawa H, Takumi N, Minegishi Y, Ohashi A, Howlader ZH, Ohsaki Y, Sato T, Goto T, Komai M
Lipids in health and disease 2011 Sep 13;10:158
Lipids in health and disease 2011 Sep 13;10:158
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of steroidogenic acute regulatory protein isoform 1 using 50 µg of mouse liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a steroidogenic acute regulatory protein isoform 1 polyclonal antibody (Product # PA5-21687) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of steroidogenic acute regulatory protein isoform 1 using 30 µg of MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a steroidogenic acute regulatory protein isoform 1 polyclonal antibody (Product # PA5-21687) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using STAR Polyclonal Antibody (Product # PA5-21687). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with STAR Polyclonal Antibody (Product # PA5-21687) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STAR was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a STAR Polyclonal Antibody (Product # PA5-21687) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using STAR Polyclonal Antibody (Product # PA5-21687). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with STAR Polyclonal Antibody (Product # PA5-21687) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAR was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a STAR Polyclonal Antibody (Product # PA5-21687) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of steroidogenic acute regulatory protein isoform 1 showing staining in the mitochondria of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a steroidogenic acute regulatory protein isoform 1 polyclonal antibody (Product # PA5-21687) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- STAR Polyclonal Antibody detects StAR protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by STAR Polyclonal Antibody (Product # PA5-21687) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- STAR Polyclonal Antibody detects StAR protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by STAR Polyclonal Antibody (Product # PA5-21687) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- STAR Polyclonal Antibody detects StAR protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. STAR Polyclonal Antibody (Product # PA5-21687) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
