Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-59587 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RBFA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DPDAPQPCGT TEPTTSSSLC GIDHEALNKQ IMEYKRRKDK GLGGLVWQGQ VAELTTQMKK GRKRAKPRLE QDSSLKSYLS GEEVEDDLDL VGAPEYECYA Highest antigen sequence identity to the following orthologs: Mouse - 42%, Rat - 48%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m4C840 12S rRNA residue.
Laptev I, Shvetsova E, Levitskii S, Serebryakova M, Rubtsova M, Zgoda V, Bogdanov A, Kamenski P, Sergiev P, Dontsova O
Nucleic acids research 2020 Aug 20;48(14):8022-8034
Nucleic acids research 2020 Aug 20;48(14):8022-8034
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBFA in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a RBFA Polyclonal Antibody (Product # PA5-59587).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RBFA in human cell line U-2 OS using a RBFA Polyclonal Antibody (Product # PA5-59587) shows localization to mitochondria.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBFA in human pancreas shows strong cytoplasmic positivity in exocrine pancreas. Samples were probed using a RBFA Polyclonal Antibody (Product # PA5-59587).