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- Antigen structure
- References [11]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-27362 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal EBI3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. This clone has been tested to be specific to the EBI3 subunit of the target protein. Clone 15K8D10 tests as being specific to the EBI3 subunit.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Interleukin-35 promotes the differentiation of regulatory T cells and suppresses Th2 response in IgG4-related type 1 autoimmune pancreatitis.
Evidence for IL-35 Expression in Diffuse Large B-Cell Lymphoma and Impact on the Patient's Prognosis.
Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion.
Expression of IL33 and IL35 in oral lichen planus.
Increased interleukin-35 expression in tumor-infiltrating lymphocytes correlates with poor prognosis in patients with breast cancer.
An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma.
Elevated level of interleukin-35 in colorectal cancer induces conversion of T cells into iTr35 by activating STAT1/STAT3.
Overexpression of interleukin-35 associates with hepatocellular carcinoma aggressiveness and recurrence after curative resection.
IL-35 expression in hepatocellular carcinoma cells is associated with tumor progression.
Tumor-derived IL-35 promotes tumor growth by enhancing myeloid cell accumulation and angiogenesis.
Human placental trophoblasts express the immunosuppressive cytokine IL-35.
Ito T, Tanaka T, Nakamaru K, Tomiyama T, Yamaguchi T, Ando Y, Ikeura T, Fukui T, Uchida K, Nishio A, Okazaki K
Journal of gastroenterology 2020 Aug;55(8):789-799
Journal of gastroenterology 2020 Aug;55(8):789-799
Evidence for IL-35 Expression in Diffuse Large B-Cell Lymphoma and Impact on the Patient's Prognosis.
Larousserie F, Kebe D, Huynh T, Audebourg A, Tamburini J, Terris B, Devergne O
Frontiers in oncology 2019;9:563
Frontiers in oncology 2019;9:563
Human placental trophoblast cells contribute to maternal-fetal tolerance through expressing IL-35 and mediating iTR35 conversion.
Liu J, Hao S, Chen X, Zhao H, Du L, Ren H, Wang C, Mao H
Nature communications 2019 Oct 10;10(1):4601
Nature communications 2019 Oct 10;10(1):4601
Expression of IL33 and IL35 in oral lichen planus.
Javvadi LR, Parachuru VPB, Milne TJ, Seymour GJ, Rich AM
Archives of dermatological research 2018 Jul;310(5):431-441
Archives of dermatological research 2018 Jul;310(5):431-441
Increased interleukin-35 expression in tumor-infiltrating lymphocytes correlates with poor prognosis in patients with breast cancer.
Zhao Z, Chen X, Hao S, Jia R, Wang N, Chen S, Li M, Wang C, Mao H
Cytokine 2017 Jan;89:76-81
Cytokine 2017 Jan;89:76-81
An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma.
Horlad H, Ma C, Yano H, Pan C, Ohnishi K, Fujiwara Y, Endo S, Kikukawa Y, Okuno Y, Matsuoka M, Takeya M, Komohara Y
Cancer science 2016 Nov;107(11):1696-1704
Cancer science 2016 Nov;107(11):1696-1704
Elevated level of interleukin-35 in colorectal cancer induces conversion of T cells into iTr35 by activating STAT1/STAT3.
Ma Y, Chen L, Xie G, Zhou Y, Yue C, Yuan X, Zheng Y, Wang W, Deng L, Shen L
Oncotarget 2016 Nov 8;7(45):73003-73015
Oncotarget 2016 Nov 8;7(45):73003-73015
Overexpression of interleukin-35 associates with hepatocellular carcinoma aggressiveness and recurrence after curative resection.
Fu YP, Yi Y, Cai XY, Sun J, Ni XC, He HW, Wang JX, Lu ZF, Huang JL, Cao Y, Zhou J, Fan J, Qiu SJ
British journal of cancer 2016 Mar 29;114(7):767-76
British journal of cancer 2016 Mar 29;114(7):767-76
IL-35 expression in hepatocellular carcinoma cells is associated with tumor progression.
Long J, Guo H, Cui S, Zhang H, Liu X, Li D, Han Z, Xi L, Kou W, Xu J, Li TS, Ding Y
Oncotarget 2016 Jul 19;7(29):45678-45686
Oncotarget 2016 Jul 19;7(29):45678-45686
Tumor-derived IL-35 promotes tumor growth by enhancing myeloid cell accumulation and angiogenesis.
Wang Z, Liu JQ, Liu Z, Shen R, Zhang G, Xu J, Basu S, Feng Y, Bai XF
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 1;190(5):2415-23
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 1;190(5):2415-23
Human placental trophoblasts express the immunosuppressive cytokine IL-35.
Mao H, Gao W, Ma C, Sun J, Liu J, Shao Q, Song B, Qu X
Human immunology 2013 Jul;74(7):872-7
Human immunology 2013 Jul;74(7):872-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.
