360904

antibody from BioLegend

Targeting: EBI3 IL27B, IL35B

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

360904

Provider

BioLegend

Proper citation

BioLegend Cat#360904, RRID:AB_2562880

Product name

PE anti-human IL-27/IL-35 EBI3 (monomer, dimer, heterodimer)

Antibody type

Monoclonal

Antigen

Purified human IL-27

Description

Epstein-Barr virus induced gene-3, also known as EBI3, is an IL-12 p40-related protein that can heterodimerize with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 diferentiation, increase cytolytic potential of CD8+ T cells, and impact B cell differentiation and Ig production.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

B032F6

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood monocytes were stimulated with IFNγ, LPS, and R848 for 24 hours (in the presence of Brefeldin A). Cells were then fixed, permeabilized, and stained with CD14 APC and EBI3 (clone B032F6) PE (top) or mouse IgG2b, κ PE isotype control (bottom).

Conjugate

Yellow dye