PA1-86790

antibody from Invitrogen Antibodies

Targeting: HAVCR1 CD365, HAVCR, HAVCR-1, KIM1, TIM-1, TIM1, TIMD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-86790

Provider

Invitrogen Antibodies

Product name

TIM-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot, this antibody detects a band at ~49kDa in human uterus tissue lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human uterine lysate probed with a TIM-1 polyclonal antibody (Product # PA1-86790) at 1 (A) and 2 (B) µg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TIM-1 was performed using 70% confluent log phase ACHN cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TIM-1 Polyclonal Antibody (Product # PA1-86790) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytosolic, Golgi localization. Panel e represents negative expression model HeLa. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.