MAB1750-100
antibody from R&D Systems
Targeting: HAVCR1
CD365, HAVCR, HAVCR-1, KIM1, TIM-1, TIM1, TIMD1
Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB1750-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TIM-1/KIM-1/HAVCR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIM-1/KIM-1/HAVCR in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAC39862
- Isotype
- IgG
- Antibody clone number
- 219211
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus.
TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection.
TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions.
Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm.
Attachment and Postattachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission.
Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication.
TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection.
Expression of Tim-1 in primary CNS lymphoma.
Acute pretreatment with chloroquine attenuates renal I/R injury in rats.
Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease.
A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
Herrador A, Fedeli C, Radulovic E, Campbell KP, Moreno H, Gerold G, Kunz S
mBio 2019 Mar 26;10(2)
mBio 2019 Mar 26;10(2)
TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection.
Niu J, Jiang Y, Xu H, Zhao C, Zhou G, Chen P, Cao R
Viruses 2018 Nov 14;10(11)
Viruses 2018 Nov 14;10(11)
TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions.
Das A, Hirai-Yuki A, González-López O, Rhein B, Moller-Tank S, Brouillette R, Hensley L, Misumi I, Lovell W, Cullen JM, Whitmire JK, Maury W, Lemon SM
mBio 2017 Sep 5;8(5)
mBio 2017 Sep 5;8(5)
Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm.
Younan P, Iampietro M, Nishida A, Ramanathan P, Santos RI, Dutta M, Lubaki NM, Koup RA, Katze MG, Bukreyev A
mBio 2017 Sep 26;8(5)
mBio 2017 Sep 26;8(5)
Attachment and Postattachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission.
Fan H, Qiao L, Kang KD, Fan J, Wei W, Luo G
Journal of virology 2017 Jul 1;91(13)
Journal of virology 2017 Jul 1;91(13)
Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication.
Aagaard KM, Lahon A, Suter MA, Arya RP, Seferovic MD, Vogt MB, Hu M, Stossi F, Mancini MA, Harris RA, Kahr M, Eppes C, Rac M, Belfort MA, Park CS, Lacorazza D, Rico-Hesse R
Scientific reports 2017 Jan 27;7:41389
Scientific reports 2017 Jan 27;7:41389
TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection.
Wang J, Qiao L, Hou Z, Luo G
Journal of virology 2017 Jan 15;91(2)
Journal of virology 2017 Jan 15;91(2)
Expression of Tim-1 in primary CNS lymphoma.
Kishimoto W, Nishikori M, Arima H, Miyoshi H, Sasaki Y, Kitawaki T, Shirakawa K, Kato T, Imaizumi Y, Ishikawa T, Ohno H, Haga H, Ohshima K, Takaori-Kondo A
Cancer medicine 2016 Nov;5(11):3235-3245
Cancer medicine 2016 Nov;5(11):3235-3245
Acute pretreatment with chloroquine attenuates renal I/R injury in rats.
Todorovic Z, Medic B, Basta-Jovanovic G, Radojevic Skodric S, Stojanovic R, Rovcanin B, Prostran M
PloS one 2014;9(3):e92673
PloS one 2014;9(3):e92673
Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease.
Wilkinson R, Wang X, Kassianos AJ, Zuryn S, Roper KE, Osborne A, Sampangi S, Francis L, Raghunath V, Healy H
PloS one 2014;9(1):e87345
PloS one 2014;9(1):e87345
A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
Kuroda M, Fujikura D, Noyori O, Kajihara M, Maruyama J, Miyamoto H, Yoshida R, Takada A
Biochemical and biophysical research communications 2014 Dec 12;455(3-4):223-8
Biochemical and biophysical research communications 2014 Dec 12;455(3-4):223-8
TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
Jemielity S, Wang JJ, Chan YK, Ahmed AA, Li W, Monahan S, Bu X, Farzan M, Freeman GJ, Umetsu DT, Dekruyff RH, Choe H
PLoS pathogens 2013 Mar;9(3):e1003232
PLoS pathogens 2013 Mar;9(3):e1003232
Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
Idali F, Wahlström J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
Respiratory research 2009 May 29;10:42
Respiratory research 2009 May 29;10:42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TIM-1/KIM-1/HAVCR by Western Blot. Western blot shows lysates of human CD4+ cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TIM-1/KIM-1/HAVCR in Human Kidney. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM-1/ KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TIM-1/KIM-1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).