PA5-79345

antibody from Invitrogen Antibodies

Targeting: HAVCR1 CD365, HAVCR, HAVCR-1, KIM1, TIM-1, TIM1, TIMD1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79345

Provider

Invitrogen Antibodies

Product name

KIM-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Deep Learning-Assisted Nephrotoxicity Testing with Bioprinted Renal Spheroids.

Tröndle K, Miotto G, Rizzo L, Pichler R, Koch F, Koltay P, Zengerle R, Lienkamp SS, Kartmann S, Zimmermann S
International journal of bioprinting 2022;8(2):528

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Paeonol Protects Against Methotrexate-Induced Nephrotoxicity via Upregulation of P-gp Expression and Inhibition of TLR4/NF-κB Pathway.

Morsy MA, El-Sheikh AAK, Abdel-Hafez SMN, Kandeel M, Abdel-Gaber SA
Frontiers in pharmacology 2022;13:774387

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Quercetin improves contrast-induced acute kidney injury through the HIF-1α/lncRNA NEAT1/HMGB1 pathway.

Luo M, Liu Z, Hu Z, He Q
Pharmaceutical biology 2022 Dec;60(1):889-898

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KIM-1 in K562 cells. Sample was incubated with KIM-1 polyclonal antibody (Product # PA5-79345).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of KIM-1 was performed using 70% confluent log phase ACHN cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KIM-1 Polyclonal Antibody (Product # PA5-79345) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytosolic, Golgi localization. Panel e represents negative expression model HeLa. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Quercetin diminished cell injury and apoptosis in the CI-AKI model via inhibiting the expression of HIF-1alpha. (A) HIF-1alpha was assessed by western blotting in different groups treated with different concentrations of quercetin. (B) Cell survival rate was gradually increased in groups treated with 10, 20, and 30 muM quercetin. (C) Cell apoptosis rate was gradually decreased in model groups with quercetin concentration increased. (D) The expression levels of IL-6 and TNF-alpha were reduced with quercetin concentration increased in the CI-AKI model. (E) The expression levels of Kim-1, NAGL, IL-6 and TNF-alpha were decreased with the concentration of quercetin increased. The data were expressed as mean +- SD. * p < 0.05, ** p < 0.01.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Quercetin diminished cell injury and apoptosis in the CI-AKI model via inhibition of HIF-1alpha on the lncRNA NEAT1/HMGB1 signalling pathway. (A, B) Relative mRNA and protein expression level of HIF-1, lncRNA NEAT1 and HMGB1 in HK-2 cells of Model, M + QC, M + QC + sh NC, M + QC + sh HIF-1alpha, M + QC + sh NC + HMGB1, and M + QC + sh HIF-1alpha + HMGB1 group was measured by qRT-PCR and western blotting. HK-2 cells were subjected for the analysis of (C) cell apoptosis rate, (D) Elisa levels of IL-6 and TNF-alpha, (E) protein expression of IL-6, TNF-alpha, Kim-1, NAGL, IL-6 and TNF-alpha between M + QC and M + QC + sh-NC, M + QC + sh-NC and M + QC + sh-HIF-1, M + QC + sh-NC + HMGB1 and M + QC + sh-HIF-1alpha+ HMGB1 group. The data were expressed as mean +- SD. * p < 0.05, ** p < 0.01.