Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BS5700 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- DNA pol ÷ 2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 144-189 of Human DNA pol ÷ 2.
- Description
- DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol Ñ´ and ÷. DNA pol Ñ´ consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol Ñ´ can be regulated by cell cycle proteins. DNA pol ÷ is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol Ñ´ and ÷ on leading and lagging strands have not yet been elucidated.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of DNA pol ¦Å 2 pAb at 1:500 dilutionLane1:MCF-7 cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate