HPA027555

antibody from Atlas Antibodies

Targeting: POLE2 DPE2

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA027555

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027555, RRID:AB_10610282

Product name

Anti-POLE2

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human POLE2, Gene description: polymerase (DNA directed), epsilon 2, accessory subunit, Alternative Gene Names: DPE2, Validated applications: ICC, Uniprot ID: P56282, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Both High-Fidelity Replicative and Low-Fidelity Y-Family Polymerases Are Involved in DNA Rereplication

Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T
Molecular and Cellular Biology 2023;35(4):699-715

---

CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis.

Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
Molecular cell 2023 Jan 5;83(1):26-42.e13

---

LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.

Fan Y, Köberlin MS, Ratnayeke N, Liu C, Deshpande M, Gerhardt J, Meyer T
The Journal of cell biology 2021 Aug 2;220(8)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & vesicles.

Sample type

Human