PA5-55654

POLE2 antibody from Invitrogen Antibodies

DPE2

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Data presented on provider website

Provider product page for PA5-55654

Antibody Data

Product number

PA5-55654

Provider

Invitrogen Antibodies

Product name

Anti-POLE2 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NAVEKQPLSS NMIERSVVEA AVQECSQSVD ETIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES GSKFQLKTIE TLLGSTTKIG DAIVLG Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 90%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
Yenerall P, Das AK, Wang S, Kollipara RK, Li LS, Villalobos P, Flaming J, Lin YF, Huffman K, Timmons BC, Gilbreath C, Sonavane R, Kinch LN, Rodriguez-Canales J, Moran C, Behrens C, Hirasawa M, Takata T, Murakami R, Iwanaga K, Chen BPC, Grishin NV, Raj GV, Wistuba II, Minna JD, Kittler R
Cell chemical biology 2020 Jan 16;27(1):105-121.e14

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLE2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a POLE2 Polyclonal Antibody (Product # PA5-55654).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLE2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a POLE2 Polyclonal Antibody (Product # PA5-55654).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of POLE2 in human cell line U-251 MG using a POLE2 Polyclonal Antibody (Product # PA5-55654) shows localization to nucleoplasm, nuclear bodies and vesicles.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of POLE2 in human cell line A-431 shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a POLE2 Polyclonal Antibody (Product # PA5-55654).