NBP1-87970

antibody from Novus Biologicals

Targeting: POLE2 DPE2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87970

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87970, RRID:AB_11060856

Product name

Rabbit Polyclonal DNA Polymerase epsilon p59 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, rat DNA Polymerase epsilon p59 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication.

Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T
Molecular and cellular biology 2015 Feb;35(4):699-715

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DNA Polymerase epsilon p59 Antibody [NBP1-87970] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DNA Polymerase epsilon p59 Antibody [NBP1-87970] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA Polymerase epsilon p59 Antibody [NBP1-87970] - Staining of human tonsil shows moderate nuclear positivity in germinal center and non-germinal center cells..