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LearnPA5-31879
antibody from Invitrogen Antibodies
Targeting: MIER3
DKFZp686L09111, DKFZp781I1119, FLJ35954
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-31879 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIER3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431. Predicted reactivity: Mouse (93%), Rat (93%), Chimpanzee (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.58 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MIER3 Polyclonal Antibody (Product # PA5-31879). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. MIER3 Polyclonal Antibody (Product # PA5-31879) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MIER3 in paraformaldehyde-fixed A431 cells using a MIER3 polyclonal antibody (Product # PA5-31879) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MIER3 Polyclonal Antibody (Product # PA5-31879) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using MIER3 Polyclonal Antibody (Product # PA5-31879) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using MIER3 Polyclonal Antibody (Product # PA5-31879) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
