MA5-24159

antibody from Invitrogen Antibodies

Targeting: CDH6

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24159

Provider

Invitrogen Antibodies

Product name

K-cadherin Monoclonal Antibody (427909)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -12, -13, -17, rhECAD, rhMCAD, rhNCAD, rhRCAD, rhPCAD, or rhVECAD is observed.

Antibody clone number

427909

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of K-cadherin in immersion fixed MG‚63 human osteosarcoma cell line. Samples were incubated in K-cadherin monoclonal antibody (Product # MA5-24159) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of MG-63 human osteosarcoma cell line was stained with mouse Anti-human Cadherin-6/KCAD Monoclonal Antibody (Product # MA5-24159, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. Cells were stained in a buffer containing Ca2+ and Mg2+.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of K-cadherin in MG‚63 human osteosarcoma cell line. Samples were incubated in K-cadherin monoclonal antibody (Product # MA5-24159) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody. Cells were stained in a buffer containing Ca2+ and Mg2+.