Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-24159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- K-cadherin Monoclonal Antibody (427909)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -12, -13, -17, rhECAD, rhMCAD, rhNCAD, rhRCAD, rhPCAD, or rhVECAD is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 427909
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of K-cadherin in immersion fixed MG‚63 human osteosarcoma cell line. Samples were incubated in K-cadherin monoclonal antibody (Product # MA5-24159) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MG-63 human osteosarcoma cell line was stained with mouse Anti-human Cadherin-6/KCAD Monoclonal Antibody (Product # MA5-24159, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. Cells were stained in a buffer containing Ca2+ and Mg2+.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of K-cadherin in MG‚63 human osteosarcoma cell line. Samples were incubated in K-cadherin monoclonal antibody (Product # MA5-24159) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody. Cells were stained in a buffer containing Ca2+ and Mg2+.
