Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF2715 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cadherin-6/KCAD Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cadherin-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-8, rhCadherin-11, and rhCadherin-12 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P55285
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
Arteriosclerosis, thrombosis, and vascular biology 2012 Jul;32(7):1724-31
Arteriosclerosis, thrombosis, and vascular biology 2012 Jul;32(7):1724-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cadherin-6/KCAD in MG-63 Human Cell Line. Cadherin-6/KCAD was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human Cadherin-6/KCAD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2715) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Cadherin-6/KCAD in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Sheep Anti-Human Cadherin-6/KCAD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2715, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).