PAB20389

antibody from Abnova Corporation

Targeting: AUP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20389

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20389, RRID:AB_10964979

Product name

AUP1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant AUP1.

Antigen sequence

LLWSLFVPFTVYQVRWLRPVHRQLGEANEEFALRV
QQLVAKELGQTGTRLTPADKAEHMKRQRHPRLRPQ
SAQSSFPPSPGPSPDVQLATLAQRVKEVLPHVPLG
VIQRDLAKTGCVDLTITNLLEGAVAFMPEDITKGT
QSLPT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with AUP1 polyclonal antibody (Cat # PAB20389).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with AUP1 polyclonal antibody (Cat # PAB20389) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, endoplasmic reticulum, vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with AUP1 polyclonal antibody (Cat # PAB20389) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)