NBP1-49916

antibody from Novus Biologicals

Targeting: AUP1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-49916

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-49916, RRID:AB_10012106

Product name

Rabbit Polyclonal Ancient ubiquitous protein 1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Detection of Human AUP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-AUP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. AUP1 was also immunoprecipitated by rabbit anti-AUP1 antibody BL10556, which recognizes an upstream epitope. For blotting immunoprecipitated AUP1 wase used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AUP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB