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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX110256 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110256, RRID:AB_1950963
- Product name
- MX1 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Bovine, Rabbit, Simian
- Host
- Rabbit
Submitted references Identification and characterization of a rabbit novel IFN-α unlocated in genome.
Characterization and signaling pathway analysis of interferon-kappa in bovine.
Suppression of USP18 Potentiates the Anti-HBV Activity of Interferon Alpha in HepG2.2.15 Cells via JAK/STAT Signaling.
Muscle myeloid type I interferon gene expression may predict therapeutic responses to rituximab in myositis patients.
Varicella Viruses Inhibit Interferon-Stimulated JAK-STAT Signaling through Multiple Mechanisms.
Cathelicidin antimicrobial peptide LL-37 in cholesteatoma enables keratinocyte reactivity with cytosolic DNA.
Methamphetamine reduces human influenza A virus replication.
Gao M, Liu Y, Guo Y, Wang Y, Dai H, Song Z, Wang J, Han W
Developmental and comparative immunology 2018 Jan;78:91-99
Developmental and comparative immunology 2018 Jan;78:91-99
Characterization and signaling pathway analysis of interferon-kappa in bovine.
Guo Y, An D, Liu Y, Bao J, Luo X, Cheng X, Wang Y, Gao M, Wang J
Developmental and comparative immunology 2017 Feb;67:213-220
Developmental and comparative immunology 2017 Feb;67:213-220
Suppression of USP18 Potentiates the Anti-HBV Activity of Interferon Alpha in HepG2.2.15 Cells via JAK/STAT Signaling.
Li L, Lei QS, Zhang SJ, Kong LN, Qin B
PloS one 2016;11(5):e0156496
PloS one 2016;11(5):e0156496
Muscle myeloid type I interferon gene expression may predict therapeutic responses to rituximab in myositis patients.
Nagaraju K, Ghimbovschi S, Rayavarapu S, Phadke A, Rider LG, Hoffman EP, Miller FW
Rheumatology (Oxford, England) 2016 Sep;55(9):1673-80
Rheumatology (Oxford, England) 2016 Sep;55(9):1673-80
Varicella Viruses Inhibit Interferon-Stimulated JAK-STAT Signaling through Multiple Mechanisms.
Verweij MC, Wellish M, Whitmer T, Malouli D, Lapel M, Jonjić S, Haas JG, DeFilippis VR, Mahalingam R, Früh K
PLoS pathogens 2015 May;11(5):e1004901
PLoS pathogens 2015 May;11(5):e1004901
Cathelicidin antimicrobial peptide LL-37 in cholesteatoma enables keratinocyte reactivity with cytosolic DNA.
Chi Z, Wang Z, Wang K, Zhu Y, Qin S
Scandinavian journal of immunology 2014 Mar;79(3):214-21
Scandinavian journal of immunology 2014 Mar;79(3):214-21
Methamphetamine reduces human influenza A virus replication.
Chen YH, Wu KL, Chen CH
PloS one 2012;7(11):e48335
PloS one 2012;7(11):e48335
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MX1 antibody [N2C2], Internal detects MX1 protein by western blot analysis. A. 30 ?g NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE MX1 antibody [N2C2], Internal (GTX110256) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MX1 antibody [N2C2], Internal detects MX1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human MX1-transfected 293T cells.7.5% SDS-PAGEMX1 antibody [N2C2], Internal (GTX110256) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MX1 antibody [N2C2], Internal detects MX1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MX1 protein stained by MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using MX1(GTX110256) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MX1 antibody [N2C2], Internal detects MX1 protein at cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. MX1 antibody [N2C2], Internal (GTX110256) diluted at 1:500.
