LS-C499388

antibody from LSBio

Targeting: MX1 IFI-78K, lncMX1-215, MxA

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C499388

Provider

LSBio

Product name

MX1 / MX Antibody LS-C499388

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive WB detected in:A549 whole cell lysate,NIH/3T3 whole cell lysate,Hela whole cell lysate;All lanes: MX1 antibody at 3ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 76,56 kDa;Observed band size: 76 kDa;

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating MX1 in Hela whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of MX1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: MX1 Antibody(4ug)+ Hela whole cell lysate(500ug);Lane 3: Hela whole cell lysate (20ug);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MX1 in Hela whole cell lysate;Lane 1: Rabbit monoclonal IgG(1ug)instead of MX1 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000);Lane 2: MX1 Antibody(4ug)+ Hela whole cell lysate(500ug);Lane 3: Hela whole cell lysate (20ug);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreatic tissue using MX1 Antibody at dilution of 1:100