Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-24914 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MX1 Monoclonal Antibody (OTI2G12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2G12
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MX1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MX1 (Product # MA5-24914) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MX1 in HeLa cells using 10 µg per lane. Samples were probed with MX1 (Product # MA5-24914) monoclonal antibody at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MX1 in 3T3 cells, mouse brain, mouse liver, mouse spleen lysate using 10 µg per lane. Samples were probed with MX1 (Product # MA5-24914) monoclonal antibody at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using MX1 Monoclonal Antibody (OTI2G12) (Product # MA5-24914) and a 91 kDa band corresponding to MX1 was observed in cell lines treated with IFN alpha. Whole cell extracts (30 µg lysate) of Daudi (Lane 1), Daudi treated with IFN Alpha (10ng/mL, 16 hrs) (Lane 2), HeLa (Lane 3) and HeLa treated with IFN Alpha (10ng/mL, 16 hrs) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX).Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177;1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MX1 in COS7 cells. Cells were transfected with a plasmid overexpressing MX1 and probed with a MX1 monoclonal antibody (Product # MA5-24914).