MA5-24914

antibody from Invitrogen Antibodies

Targeting: MX1 IFI-78K, lncMX1-215, MxA

Antibody data

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Validation data

MX1 protein structure - MA5-24914 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MX1 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MX1 (Product # MA5-24914) monoclonal antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MX1 in HeLa cells using 10 µg per lane. Samples were probed with MX1 (Product # MA5-24914) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MX1 in 3T3 cells, mouse brain, mouse liver, mouse spleen lysate using 10 µg per lane. Samples were probed with MX1 (Product # MA5-24914) monoclonal antibody at a dilution of 1:200.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using MX1 Monoclonal Antibody (OTI2G12) (Product # MA5-24914) and a 91 kDa band corresponding to MX1 was observed in cell lines treated with IFN alpha. Whole cell extracts (30 µg lysate) of Daudi (Lane 1), Daudi treated with IFN Alpha (10ng/mL, 16 hrs) (Lane 2), HeLa (Lane 3) and HeLa treated with IFN Alpha (10ng/mL, 16 hrs) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX).Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177;1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).