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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-42620 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Hemoglobin gamma-1 Recombinant Rabbit Monoclonal Antibody (6F1A8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: Mouse kidney. Immunogen sequence: MGHFTEEDKA TITSLWGKVN VEDAGGETLG RLLVVYPWTQ RFFDSFGNLS SASAIMGNPK VKAHGKKVLT SLGDAIKHLD DLKGTFAQLS ELHCDKLHVD
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 6F1A8
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Hemoglobin gamma-1 in Mouse kidney cells using a Hemoglobin gamma-1 Recombinant Monoclonal antibody (Product # MA5-42620) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Hemoglobin gamma-1 in human placenta using a Hemoglobin gamma-1 Recombinant Monoclonal antibody (Product # MA5-42620). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Hemoglobin gamma-1 in human placenta using a Recombinant Monoclonal antibody (Product # MA5-42620) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
