AP33458PU-N

antibody from Acris Antibodies GmbH

Targeting: GFRA4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP33458PU-N

Provider

Acris Antibodies GmbH

Product name

anti GFRA4 / GDNFR-alpha 4

Antibody type

Polyclonal

Antigen

Recombinant His-tagged human GFRa-4 protein produced using CHO-based. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

Reactivity

Human

Host

Chicken/Avian

Isotype

IgY

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Lane 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Lane 2. Prestained protein ladder (Naxo 8003). Rabbit anti-chicken antibody, HRP conjugate was used as secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

IF detection of human GFRα-4 expressed in U2OS cells. GFRα-4 was visualized using Chicken polyclonal antibody to human GFRα-4 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-4 specific signal and blue for nuclei.