Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB10041-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse ST2/IL-33R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse ST2/IL-33 R in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P14719
- Isotype
- IgG
- Antibody clone number
- 245707
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice.
Mast cells are essential intermediaries in regulating IL-33/ST2 signaling for an immune network favorable to mucosal healing in experimentally inflamed colons.
IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy.
CD301b⁺ dermal dendritic cells drive T helper 2 cell-mediated immunity.
Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.
Ito Y, Schaefer N, Sanchez A, Francisco D, Alam R, Martin RJ, Ledford JG, Stevenson C, Jiang D, Li L, Kraft M, Chu HW
Journal of innate immunity 2018;10(2):106-118
Journal of innate immunity 2018;10(2):106-118
Mast cells are essential intermediaries in regulating IL-33/ST2 signaling for an immune network favorable to mucosal healing in experimentally inflamed colons.
He Z, Song J, Hua J, Yang M, Ma Y, Yu T, Feng J, Liu B, Wang X, Li Y, Li J
Cell death & disease 2018 Dec 5;9(12):1173
Cell death & disease 2018 Dec 5;9(12):1173
IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy.
Liu B, Tai Y, Achanta S, Kaelberer MM, Caceres AI, Shao X, Fang J, Jordt SE
Proceedings of the National Academy of Sciences of the United States of America 2016 Nov 22;113(47):E7572-E7579
Proceedings of the National Academy of Sciences of the United States of America 2016 Nov 22;113(47):E7572-E7579
CD301b⁺ dermal dendritic cells drive T helper 2 cell-mediated immunity.
Kumamoto Y, Linehan M, Weinstein JS, Laidlaw BJ, Craft JE, Iwasaki A
Immunity 2013 Oct 17;39(4):733-43
Immunity 2013 Oct 17;39(4):733-43
Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
Monticelli LA, Sonnenberg GF, Abt MC, Alenghat T, Ziegler CG, Doering TA, Angelosanto JM, Laidlaw BJ, Yang CY, Sathaliyawala T, Kubota M, Turner D, Diamond JM, Goldrath AW, Farber DL, Collman RG, Wherry EJ, Artis D
Nature immunology 2011 Nov;12(11):1045-54
Nature immunology 2011 Nov;12(11):1045-54
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.
Ohno T, Oboki K, Morita H, Kajiwara N, Arae K, Tanaka S, Ikeda M, Iikura M, Akiyama T, Inoue J, Matsumoto K, Sudo K, Azuma M, Okumura K, Kamradt T, Saito H, Nakae S
PloS one 2011 Apr 11;6(4):e18404
PloS one 2011 Apr 11;6(4):e18404
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ST2/IL-33 R in P815 Mouse Cell Line by Flow Cytometry. P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL-33 induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041). The ND50 is typically 0.5-3 μg/mL.