MAB523-100

antibody from R&D Systems

Targeting: IL1RL1 DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB523-100

Provider

R&D Systems

Product name

Human ST2/IL-33R Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human ST2/IL-33 R in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse ST2L, recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 RA, rhIL-1 alpha , or rhIL-1 beta is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

BAA02233


Isotype

IgG

Antibody clone number

97203

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Interleukin-33 Predicts Poor Prognosis and Promotes Renal Cell Carcinoma Cell Growth Through its Receptor ST2 and the JNK Signaling Pathway.

Wu CW, Wu YG, Cheng C, Hong ZD, Shi ZM, Lin SQ, Li J, He XY, Zhu AY
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;47(1):191-200

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From proteomics to discovery of first-in-class ST2 inhibitors active in vivo.

Ramadan AM, Daguindau E, Rech JC, Chinnaswamy K, Zhang J, Hura GL, Griesenauer B, Bolten Z, Robida A, Larsen M, Stuckey JA, Yang CY, Paczesny S
JCI insight 2018 Jul 26;3(14)

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A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis.

Díaz-Jiménez D, Núñez L, De la Fuente M, Dubois-Camacho K, Sepúlveda H, Montecino M, Torres-Riquelme A, García-González P, Chnaiderman J, Vossenkamper A, MacDonald TT, Simian D, González MJ, Cidlowski JA, Quera R, Hermoso MA
Scientific reports 2017 Aug 31;7(1):10180

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Daily exposure to dust alters innate immunity.

Sahlander K, Larsson K, Palmberg L
PloS one 2012;7(2):e31646

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IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.

Blom L, Poulsen LK
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4331-7

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The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis.

Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Feb;42(2):218-28

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Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.

Yasuo T, Kitaya K
Fertility and sterility 2009 Aug;92(2):709-21

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IFN-gamma Secretion Induced by IL-33 and Neutralization by Human ST2/IL-33 R Antibody. Recombinant Human IL-33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND₅₀ is typically 0.3-1.5 μg/mL.