Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1004 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ST2/IL-33R Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ST2/IL-33 R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice.
α-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
Ito Y, Schaefer N, Sanchez A, Francisco D, Alam R, Martin RJ, Ledford JG, Stevenson C, Jiang D, Li L, Kraft M, Chu HW
Journal of innate immunity 2018;10(2):106-118
Journal of innate immunity 2018;10(2):106-118
α-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3.
Huang JR, Tsai YC, Chang YJ, Wu JC, Hung JT, Lin KH, Wong CH, Yu AL
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1972-81
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1972-81
Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
Mizutani N, Nabe T, Yoshino S
Immunology 2013 Jun;139(2):205-18
Immunology 2013 Jun;139(2):205-18
IL-33 is produced by mast cells and regulates IgE-dependent inflammation.
Hsu CL, Neilsen CV, Bryce PJ
PloS one 2010 Aug 3;5(8):e11944
PloS one 2010 Aug 3;5(8):e11944
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
Protective effect of IL-18 on kainate- and IL-1 beta-induced cerebellar ataxia in mice.
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2322-8
ST2 is essential for Th2 responsiveness and resistance to pseudomonas aeruginosa keratitis.
Huang X, Du W, Barrett RP, Hazlett LD
Investigative ophthalmology & visual science 2007 Oct;48(10):4626-33
Investigative ophthalmology & visual science 2007 Oct;48(10):4626-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL-33 (Catalog # 3625-IL) induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-Mouse ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1004). The ND50 is typically 1-6 μg/mL.