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antibody from Invitrogen Antibodies
Targeting: IL1RL1
DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-47024 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ST2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) ST2/IL-1 R4 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of KG-1a human acute myelogenous leukemia cell line was stained with Goat Anti-human ST2/IL-1 R4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47024, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization assay of recombinant human IL-33 induces IFN-γ secretion in human Peripheral Blood Mononuclear cells (PBMC) in the presence of 0.25 ng/mL recombinant human IL-12 in a dose-dependent manner (orange line), as measured by the human IFN-γ Q-kit. Under these conditions, IFN-γ secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Goat Anti-human ST2/IL-1 R4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47024). The ND50 is typically 0.1-0.6 µg/mL.
