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Learn500-P261BT-1MG
antibody from Invitrogen Antibodies
Targeting: IL1RL1
DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P261BT-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-33 Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-33. Anti-Human IL-33-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hIL-33 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-33 (500-P261) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P261BT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 2 x 500 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hIL-33 by Western Blot analysis IL-33 Polyclonal Antibody, Biotin (Product # 500-P261BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hIL-33 by Western Blot analysis IL-33 Polyclonal Antibody, Biotin (Product # 500-P261BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect hIL-33 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL-33 Polyclonal Antibody, Biotin (Product # 500-P261BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-33 Polyclonal Antibody (Product # 500-P261-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-33.
- Conjugate
- Biotin
