PA5-28383

antibody from Invitrogen Antibodies

Targeting: IL1RL1 DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1

Antibody data

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Validation data

IL1RL1 protein structure - PA5-28383 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ST2 using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ST2 polyclonal antibody (Product # PA5-28383) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ST2 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ST2 Polyclonal Antibody (Product # PA5-28383) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ST2 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ST2 Polyclonal Antibody (Product # PA5-28383) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ST2 Polyclonal Antibody (Product # PA5-28383) and a ~63kDa band corresponding to Interleukin-1 receptor-like 1 was observed across iPSCs. Membrane enriched extracts (50 µg lysate) of iPSC (Lane 1), iPSC differentiated to cardiomyocytes (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Enhanced signal was observed in iPSCs differentiated into cardiomyocytes as compared to in undifferentiated iPSCs.