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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- A300-680A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GbetaL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse GbetaL by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GbetaL antibody (Product # A300-680A; lot A300-680A-3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human GbetaL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GbetaL antibody A300-680A (lot A300-680A-2) used for IP at 6 µg per reaction. GbetaL was also immunoprecipitated by rabbit anti-GbetaL antibody A300-679A. For blotting immunoprecipitated GbetaL, A300-680A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse GbetaL by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GbetaL antibody A300-680A (lot A300-680A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
