Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF840 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-18R alpha/IL-1 R5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-18 R alpha/IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-18 R alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation.
Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma.
Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.
Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
Gibbs A, Leeansyah E, Introini A, Paquin-Proulx D, Hasselrot K, Andersson E, Broliden K, Sandberg JK, Tjernlund A
Mucosal immunology 2017 Jan;10(1):35-45
Mucosal immunology 2017 Jan;10(1):35-45
Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma.
Lin L, Wang J, Liu D, Liu S, Xu H, Ji N, Zhou M, Zeng X, Zhang D, Li J, Chen Q
Scientific reports 2016 May 26;6:26757
Scientific reports 2016 May 26;6:26757
Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
Dusseaux M, Martin E, Serriari N, Péguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
Blood 2011 Jan 27;117(4):1250-9
Blood 2011 Jan 27;117(4):1250-9
Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.
Sallmon H, Hoene V, Weber SC, Dame C
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2010 Feb;30(2):55-8
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2010 Feb;30(2):55-8
Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Bührer C
Annals of neurology 2005 Jan;57(1):50-9
Annals of neurology 2005 Jan;57(1):50-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-18 R alpha/IL-1 R5 in Human Skin. IL-18 R alpha/IL-1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL-18 R alpha/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Human IL-18 R alpha/IL-1 R5 Antibody. Recombinant Human IL-18/IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). IFN-gamma secretion elicited by IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 R alpha/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.