Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB12161-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-18 R alpha/IL-1 R5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, rhIL-1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q61098- Isotype
- IgG
- Antibody clone number
- 112624
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter.
The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
Protection of CD8+ T cells from activation-induced cell death by IL-18.
IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi MA, He A, Zhou Y, Sukhova GK, Chen H, Cheng XW, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull GE, Rogers S, Yang CL, Ke Q, Jelen S, Bindels R, Ellison DH, Jarolim P, Libby P, Shi GP
Nature medicine 2015 Jul;21(7):820-6
Nature medicine 2015 Jul;21(7):820-6
The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 1;187(3):1333-46
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 1;187(3):1333-46
Protection of CD8+ T cells from activation-induced cell death by IL-18.
Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
Journal of leukocyte biology 2007 Jul;82(1):142-51
Journal of leukocyte biology 2007 Jul;82(1):142-51
IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation.
Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1948-59
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1948-59
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutraliza-tion by IL-18 R alpha/IL-1 R5 Antibody. In the presence of Recombinant Mouse IL-12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL-18/IL-1F4 stimulates IFN-gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN-gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN-gamma secretion elicited by Recombinant Mouse IL-18/IL-1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-18 R alpha/IL-1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND50 is typically 0.2-0.8 µg/mL.
