MAB1216

antibody from R&D Systems

Targeting: IL18R1 CD218a, IL-1Rrp, IL1RRP

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1216

Provider

R&D Systems

Product name

Mouse IL-18 R alpha/IL-1 R5 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-18 R alpha/IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, recombinant human (rh) IL-1 R3, rhIL-1 R4, recombinant rat (rr) IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q61098


Isotype

IgG

Antibody clone number

112614

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Mapping of the full length and the truncated interleukin-18 receptor alpha in the mouse brain.

Alboni S, Cervia D, Ross B, Montanari C, Gonzalez AS, Sanchez-Alavez M, Marcondes MC, De Vries D, Sugama S, Brunello N, Blom J, Tascedda F, Conti B
Journal of neuroimmunology 2009 Sep 29;214(1-2):43-54

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-18 R alpha/IL-1 R5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL-18 R alpha/IL-1 R5 Monoclonal Antibody (Catalog # MAB1216, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂ Secondary Antibody (Catalog # F0105B).