Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB840P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-18 R alpha/IL-1 R5 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-18 R alpha/IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q13478
- Isotype
- IgG
- Antibody clone number
- 70625
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-γ.
Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression.
Increased expression of interleukin-18 receptor in blood cells of subjects with mild cognitive impairment and Alzheimer's disease.
Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
Sundström P, Ahlmanner F, Akéus P, Sundquist M, Alsén S, Yrlid U, Börjesson L, Sjöling Å, Gustavsson B, Wong SB, Quiding-Järbrink M
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3472-81
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3472-81
Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression.
Uzan B, Poglio S, Gerby B, Wu CL, Gross J, Armstrong F, Calvo J, Cahu X, Deswarte C, Dumont F, Passaro D, Besnard-Guérin C, Leblanc T, Baruchel A, Landman-Parker J, Ballerini P, Baud V, Ghysdael J, Baleydier F, Porteu F, Pflumio F
EMBO molecular medicine 2014 Jun;6(6):821-34
EMBO molecular medicine 2014 Jun;6(6):821-34
Increased expression of interleukin-18 receptor in blood cells of subjects with mild cognitive impairment and Alzheimer's disease.
Salani F, Ciaramella A, Bizzoni F, Assogna F, Caltagirone C, Spalletta G, Bossù P
Cytokine 2013 Feb;61(2):360-3
Cytokine 2013 Feb;61(2):360-3
Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
Dusseaux M, Martin E, Serriari N, Péguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
Blood 2011 Jan 27;117(4):1250-9
Blood 2011 Jan 27;117(4):1250-9
Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
de Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ
Arthritis and rheumatism 2009 Sep;60(9):2782-93
Arthritis and rheumatism 2009 Sep;60(9):2782-93
IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5571-7
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5571-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of IL-18 R alpha/IL-1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 5 μg/mL PHA for 48 hours were stained with Mouse Anti-Human IL-18 R alpha/IL-1 R5 PE-conjugated Monoclonal Antibody (Catalog # FAB840P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.