Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-7183-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD218a (IL-18Ra) Monoclonal Antibody (H44), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The H44 antibody reacts with the interleukin-18 receptor alpha chain, also known as IL-1Rrp, an IL-1R family member. IL-18R alpha is expressed on Th1 cells, NK cell subset, neutrophils and IL-12-stimulated tonsillar B cells. By itself, IL-18R alpha binds IL-18 with low affinity and when complexed with IL-18R beta chain binds IL-18 with high affinity. The proinflammatory cytokine IL-18 and its receptor complex play an important role in the innate and autoimmune responses. Applications Reported: H44 has been reported for use in flow cytometric analysis. Applications Tested: This H44 antibody has been pre-titrated and tested by flow cytometric analysis on human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to stain whole blood before RBC lysis. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- H44
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references IL-18 Is Involved in Eosinophil-Mediated Tumoricidal Activity against a Colon Carcinoma Cell Line by Upregulating LFA-1 and ICAM-1.
Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.
Human Umbilical Cord-Derived Mesenchymal Stem Cells Utilize Activin-A to Suppress Interferon-γ Production by Natural Killer Cells.
Stress-related and homeostatic cytokines regulate Vγ9Vδ2 T-cell surveillance of mevalonate metabolism.
Gatault S, Delbeke M, Driss V, Sarazin A, Dendooven A, Kahn JE, Lefèvre G, Capron M
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2483-92
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 1;195(5):2483-92
Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.
Tsai CY, Liong KH, Gunalan MG, Li N, Lim DS, Fisher DA, MacAry PA, Leo YS, Wong SC, Puan KJ, Wong SB
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3890-900
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3890-900
Human Umbilical Cord-Derived Mesenchymal Stem Cells Utilize Activin-A to Suppress Interferon-γ Production by Natural Killer Cells.
Chatterjee D, Marquardt N, Tufa DM, Hatlapatka T, Hass R, Kasper C, von Kaisenberg C, Schmidt RE, Jacobs R
Frontiers in immunology 2014;5:662
Frontiers in immunology 2014;5:662
Stress-related and homeostatic cytokines regulate Vγ9Vδ2 T-cell surveillance of mevalonate metabolism.
Gruenbacher G, Nussbaumer O, Gander H, Steiner B, Leonhartsberger N, Thurnher M
Oncoimmunology 2014;3(8):e953410
Oncoimmunology 2014;3(8):e953410
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD218a (IL-18 Receptor alpha) PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye