AF840

antibody from R&D Systems

Targeting: IL18R1 CD218a, IL-1Rrp, IL1RRP

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF840

Provider

R&D Systems

Product name

Human IL-18 R alpha/IL-1 R5 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human IL-18 R alpha/IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-18 R alpha is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q13478


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation.

Gibbs A, Leeansyah E, Introini A, Paquin-Proulx D, Hasselrot K, Andersson E, Broliden K, Sandberg JK, Tjernlund A
Mucosal immunology 2017 Jan;10(1):35-45

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Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma.

Lin L, Wang J, Liu D, Liu S, Xu H, Ji N, Zhou M, Zeng X, Zhang D, Li J, Chen Q
Scientific reports 2016 May 26;6:26757

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Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.

Dusseaux M, Martin E, Serriari N, Péguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
Blood 2011 Jan 27;117(4):1250-9

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Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.

Sallmon H, Hoene V, Weber SC, Dame C
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2010 Feb;30(2):55-8

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Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.

Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Bührer C
Annals of neurology 2005 Jan;57(1):50-9

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-18 R alpha/IL-1 R5 in Human Skin. IL-18 R alpha/IL-1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL-18 R alpha/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Human IL-18 R alpha/IL-1 R5 Antibody. Recombinant Human IL-18/IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). IFN-gamma secretion elicited by IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 R alpha/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND₅₀ is typically 0.1-0.4 µg/mL.